Analytical Data
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Gene name
ERK3
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简介
ERK3 Protein, an atypical MAPK, phosphorylates MAP2 and MAPKAPK5. Interacting with MAPKAPK5, ERK3/MAPK6 undergoes Ser-189 phosphorylation, facilitating MAPKAPK5 activation. Activated MAPKAPK5 reciprocally phosphorylates ERK3/MAPK6. The complex's precise functional implications, especially in cell cycle entry, remain elusive. ERK3 Protein, Human (L290V, His) is the recombinant human-derived ERK3 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
MAPK6; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16659
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Expression Region
M9-I327, L290V
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ERK3 (Extracellular Signal-Regulated Kinase 3) is a member of the MAPK family that plays a crucial role in various cellular processes, including cell differentiation, proliferation, and survival. Unlike its more extensively studied counterparts in the ERK subfamily, ERK1 and ERK2, ERK3 has garnered attention due to its unique regulatory mechanisms and functions. Research indicates that ERK3 is involved in the regulation of several cellular pathways, including those linked to stress responses and developmental processes. Additionally, ERK3 has been implicated in certain pathological conditions, including cancer, where it may influence tumor progression and cell cycle regulation. The production of recombinant ERK3 protein allows for detailed biophysical and biochemical studies, enabling researchers to explore its interactions, activation, and downstream effects in various cellular contexts. Understanding the molecular mechanisms of ERK3 is essential for elucidating its potential as a therapeutic target. As interest grows in the role of unconventional MAPKs in health and disease, recombinant ERK3 protein is poised to provide valuable insights into its biological relevance and potential applications in targeted therapies.
