Analytical Data
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Gene name
Kirrel1/NEPH1
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简介
The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier, helping to maintain a stable podocyte structure and interdigitated foot processes connected by specialized cell-to-cell junctions called slit septa. Additionally, it acts as a signaling protein that requires the presence of TEC kinase to fully transactivate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Nephrin-like protein 1; Kirrel1; Neph1
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Species
Mouse
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q80W68-1
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Expression Region
L48-L525
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AA Sequence
LPGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAIPNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVYNKVGSTNVSTLVNVHFAPRIVVYPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQFAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVL
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Protein Length
Partial
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Molecular Weight
60-90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Kirrel1, also known as Nephrin-like protein 1 (NEPH1), is a member of the immunoglobulin superfamily that plays a pivotal role in the development and maintenance of the kidney's filtration barrier. Its significance became apparent through studies linking mutations in the Kirrel1/NEPH1 gene to congenital nephrotic syndrome, a condition characterized by proteinuria and edema due to the faulty filtration process in the kidneys. Research on Kirrel1/NEPH1 focuses on understanding its molecular mechanisms in podocyte biology—the specialized cells that form part of the glomerular filtration barrier. The protein is known to participate in cell-cell adhesion and signaling pathways that contribute to podocyte stability and function. Given its critical role in kidney health, Kirrel1/NEPH1 is a target of interest for therapeutic development in kidney diseases. Studies involving recombinant Kirrel1/NEPH1 proteins aim to elucidate its structural properties, interaction with other proteins, and potential functions in cellular signaling. Investigating Kirrel1/NEPH1 may provide insights into novel approaches for treating kidney disorders, as well as improve our understanding of the underlying pathophysiological processes in renal diseases. The ongoing research in this area not only enhances our knowledge of kidney biology but also paves the way for the development of innovative strategies to combat kidney-related ailments.
