Analytical Data
-
Gene name
ATG1
- Application
-
Alternative Names
APG10L; Ubiquitin-like-conjugating enzyme ATG10
-
Species
Rat
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 95% as determined by SDS-PAGE.
-
Uniprot
D4A9K6
-
Expression Region
Met1~Glu214
-
Molecular Weight
30kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATG1, or Autophagy-related gene 1, plays a crucial role in the initiation of autophagy, a vital cellular process that degrades and recycles cellular components. This protein is a serine/threonine kinase involved in the regulation of autophagic pathways, particularly in response to nutrient deprivation and stress conditions. The study of ATG1 has gained significant attention in recent years due to its implications in various biological processes and diseases, including neurodegeneration, cancer, and infectious diseases. Research has demonstrated that ATG1 is essential for the formation of the phagophore, the precursor to autophagosomes, and that its activity is regulated by various cellular signals, including mTOR and AMPK pathways. Understanding the function and regulation of ATG1 is critical for elucidating the mechanisms of autophagy and its impact on cellular homeostasis. Moreover, the potential therapeutic targeting of ATG1 and its downstream autophagic processes offers promising avenues for treating diseases where autophagy is dysregulated. Further characterization and development of recombinant ATG1 proteins will enhance our understanding of autophagy and potentially lead to innovative treatments for related disorders. Researchers are focusing on engineering ATG1 for improved functionality and stability, paving the way for future studies on its interactions within the autophagy machinery. The identification of ATG1's structural features and regulatory mechanisms will contribute significantly to the broader field of cell biology and therapeutic interventions in autophagy-related diseases.
