Analytical Data
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Gene name
GADD45B
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简介
GADD45B Protein, a pivotal mediator, regulates growth and apoptosis by activating the MTK1/MEKK4 MAPKKK signaling pathway. Interacting with GADD45GIP1, it forms a complex that modulates cellular responses under stress. Its central role in growth and apoptosis underscores its significance in orchestrating cellular processes, positioning GADD45B as a key player in the intricate network of signaling pathways governing cell fate decisions. GADD45B Protein, Human (His) is the recombinant human-derived GADD45B protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75293
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Expression Region
M1-R160
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Protein Length
Full Length
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Molecular Weight
19 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GADD45B (Growth Arrest and DNA Damage-inducible protein 45 beta) is a member of the GADD45 family, which is known to be involved in cellular stress responses, DNA repair, and apoptosis. Research on GADD45B has gained attention due to its potential role in cancer biology and other diseases, as well as its regulatory influence on cell proliferation and survival. The expression of GADD45B is often upregulated in response to various stressors, including oxidative stress, UV irradiation, and DNA damage, indicating its involvement in the cellular defense mechanism. Additionally, studies have suggested that GADD45B may act as a tumor suppressor, with its downregulation contributing to oncogenesis in several cancer types. Understanding the function and regulatory mechanisms of GADD45B at a molecular level is crucial for elucidating its role in disease pathogenesis and for exploring its potential as a therapeutic target. The generation of recombinant GADD45B protein has facilitated in-depth investigations into its biochemical properties and interactions with other cellular molecules, paving the way for novel therapeutic strategies aimed at modulating its activity to combat cancer and other stress-related diseases. The ongoing research efforts to characterize GADD45B's functions and the pathways it influences are promising, potentially leading to significant advancements in targeted treatments and improved understanding of tumor biology.
