Analytical Data
-
Gene name
HK3/Hexokinase-3
-
简介
The HK3 (hexokinase-3) protein promotes the phosphorylation of hexoses (i.e., D-glucose and D-fructose) to form hexose-6-phosphate (specifically, D-glucose 6-phosphate and 6-phosphate), Plays a vital role in cell metabolism. D-fructose 6-phosphate. HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is the recombinant human-derived HK3/Hexokinase-3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
-
Alternative Names
Hexokinase-3; HK III; Hexokinase-C; HK3
-
Species
Human
-
Source
Baculovirus
-
Tag
N-His;N-GST
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P52790
-
Expression Region
M1-V923
-
Protein Length
Full Length
-
Molecular Weight
115 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HK3, or Hexokinase-3, is an enzyme that plays a crucial role in glucose metabolism by phosphorylating glucose to form glucose-6-phosphate, a key step in both glycolysis and cellular energy production. The study of HK3 is particularly significant due to its implications in various physiological and pathological processes, including cancer metabolism, diabetes, and neurodegenerative diseases. Unlike other isoforms of hexokinase, HK3 is less understood but appears to exhibit unique regulatory mechanisms and tissue distribution, prompting interest in its potential as a therapeutic target. Research into the recombinant expression of HK3 aims to provide insight into its structure-function relationship, regulatory pathways, and interaction with other metabolic enzymes. By producing HK3 as a recombinant protein, scientists can conduct detailed biochemical studies, structural analyses, and drug screening assays to develop targeted therapies. Understanding HK3's role in metabolic pathways could lead to novel approaches for managing metabolic disorders and enhancing cellular energy efficiency. The ongoing exploration of HK3 not only enriches our knowledge of cellular metabolism but also opens avenues for innovative treatments in metabolic diseases.
