Analytical Data
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基因名
Propionate kinase/TdcD
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简介
Propionate kinase, also known as TdcD protein, plays a key role in cellular metabolism by catalyzing the conversion of propionyl phosphate and ADP into propionate and ATP. This enzymatic process is an integral part of the metabolic pathways associated with propionate utilization, facilitating the conversion of propionyl phosphate into propionate, a compound essential for various cellular functions. Propionate kinase/TdcD Protein, E.coli (Myc, His) is the recombinant E. coli-derived Propionate kinase/TdcD protein, expressed by E. coli , with N-His, C-Myc labeled tag.
- Application
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别名
tdcD; c3873; Propionate kinase; EC 2.7.2.15
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种属
E.coli
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表达系统
E. coli
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标签
N-His;C-Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P59244
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表达区间
M1-A402
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氨基酸序列
MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAFLSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITDEVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLPWKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQSVDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLSSDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLIRRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHLGKVNAPAEFA
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Propionate kinase, also known as TdcD, is an enzyme that plays a critical role in the metabolic pathway of propionate, a short-chain fatty acid that is important for various biological processes. It catalyzes the conversion of propionate into propionyl phosphate, which is an essential step in energy production, especially in anaerobic bacteria, where fermentation processes are key to survival. The study of TdcD has garnered attention due to its potential applications in biotechnology and medicine, particularly in metabolic engineering and the development of biosynthetic pathways for the production of valuable compounds. Understanding the structure and function of the recombinant TdcD protein can provide insights into its enzymatic mechanisms and regulatory roles. Furthermore, the manipulation of this enzyme may lead to advancements in microbial fermentation technologies, enhancing the efficiency of production systems that utilize propionate as a substrate. Due to the rising interest in sustainable production methods and the need for innovative solutions in bioengineering, research on propionate kinase/TdcD is significant for both academic and practical applications, paving the way for new strategies in the production of biofuels, bioplastics, and other industrially relevant biochemicals. Overall, delving into the properties and functionality of recombinant TdcD holds promise not only for understanding fundamental biochemical processes but also for advancing the field of synthetic biology.
