Analytical Data
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Gene name
PMF1
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简介
The PMF1 protein is part of the MIS12 complex and ensures the correct alignment, segregation and kinetochore formation of chromosomes in mitosis. It also acts as a co-transcriptional partner of NFE2L2, regulating polyamine-induced SSAT transcription. PMF1 Protein, Human (His, Strep) is the recombinant human-derived PMF1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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Alternative Names
PMF1; Polyamine-modulated factor 1; PMF-1
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P1K2
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Expression Region
M1-E205
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PMF1, or protein of unknown function 1, is a recently identified protein that has garnered attention in the field of molecular biology due to its potential roles in cellular processes and disease mechanisms. Research into PMF1 began as part of broader efforts to understand the functions of uncharacterized proteins that represent a significant portion of the proteome. Its expression patterns have been observed in various tissues, suggesting it may play a role in development and cellular signaling pathways. Furthermore, preliminary studies indicate that PMF1 could be involved in stress responses and may have implications in certain pathological conditions, such as cancer or neurodegenerative diseases. As a result, elucidating the structure and function of PMF1 through recombinant protein studies is crucial. These studies aim to not only determine its biochemical properties but also to explore its interactions with other cellular components. By employing techniques such as gene cloning, expression in model systems, and functional assays, researchers hope to unveil the biological significance of PMF1. Understanding PMF1's role could provide valuable insights into fundamental biological processes and might also open up new avenues for therapeutic interventions in diseases linked to its dysfunction. The ongoing research into PMF1 exemplifies the importance of investigating uncharacterized proteins, which could yield significant advancements in cell biology and medicine.
