Analytical Data
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Gene name
GEMIN6
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简介
The GEMIN6 protein is a component of the SMN complex and is critical for the assembly of small nuclear ribonucleoproteins (snRNPs), which are essential for spliceosome-mediated pre-mRNA splicing. Most spliceosomal snRNPs share a common set of Sm proteins that form a heptameric protein ring on small nuclear RNAs. GEMIN6 Protein, Human (His, Strep) is the recombinant human-derived GEMIN6 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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Alternative Names
GEMIN6; Gem-associated protein 6; Gemin-6; SIP2
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WXD5
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Expression Region
M1-Q167
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GEMIN6, a key component of the survival of motor neuron (SMN) complex, plays a critical role in the biogenesis and maintenance of small nuclear ribonucleoproteins (snRNPs), which are essential for mRNA splicing. Research into GEMIN6 has garnered interest due to its involvement in spinal muscular atrophy (SMA), a genetic disorder characterized by the degeneration of motor neurons. The SMN complex, which includes GEMIN6, is vital for RNA metabolism and cellular functions, with its dysfunction leading to the pathology observed in SMA patients. Additionally, GEMIN6 has been implicated in various cellular processes beyond snRNP assembly, including the regulation of gene expression and cellular stress responses. Understanding the precise mechanisms by which GEMIN6 operates and its interactions with other SMN complex components can provide insights into potential therapeutic strategies for SMA and other related neurodegenerative diseases. The recombinant expression and characterization of GEMIN6 are crucial for elucidating its structure-function relationships, allowing researchers to explore its role in health and disease and the potential for targeted interventions.
