Analytical Data
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基因名
KBTBD7
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简介
KBTBD7 is a component of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex and acts as a substrate linker to direct “Lys-48” ubiquitination and subsequent proteasomal degradation of the RAC1 guanine exchange factor TIAM1. This precise ubiquitination enables KBTBD7 to effectively regulate RAC1 signaling, affecting key biological processes such as cytoskeletal organization, cell migration, and cell proliferation. KBTBD7 Protein, Human (sf9, His, Strep) is the recombinant human-derived KBTBD7 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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别名
KBTBD7; Kelch repeat and BTB domain-containing protein 7
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种属
Human
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表达系统
Baculovirus
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标签
N-StrepⅡ;N-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8WVZ9
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表达区间
Q2-L684
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KBTBD7 is a member of the Kelch repeat and BTB (bric-a-brac, tramtrack, and broad complex) domain-containing protein family, which plays crucial roles in various cellular processes, including protein ubiquitination and degradation. Research on KBTBD7 has gained momentum due to its potential implications in cancer biology, neurodegenerative diseases, and cellular stress responses. Studies have suggested that KBTBD7 may act as a substrate adaptor for the cullin-RING E3 ubiquitin ligase complex, facilitating the ubiquitination of specific target proteins, thereby influencing their stability and function. Furthermore, altered expression or mutations in KBTBD7 have been linked to various pathological conditions, underscoring its relevance in disease mechanisms. Understanding the structure and function of KBTBD7, particularly through the study of recombinant protein forms, can illuminate its biological roles and regulatory mechanisms. This research holds promise for uncovering novel therapeutic targets and strategies, especially in the context of malignancies where dysregulated protein turnover is often a hallmark. The investigation into KBTBD7's interactions and modifications could further elucidate its integration within signaling pathways and cellular homeostasis, paving the way for advances in biomedical applications and therapies.
