Analytical Data
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Gene name
T4 ligase
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简介
The T4 ligase protein is expressed during the early stages of lytic development and serves as a DNA ligase involved in various aspects of T4 phage biology. It plays a crucial role in T4 DNA synthesis, promoting the formation of phosphodiester bonds to join DNA fragments. T4 ligase Protein, T4 phage (His) is the recombinant T4 ligase protein, expressed by E. coli , with N-6*His labeled tag. T4 ligase Protein, T4 phage (His), has molecular weight of ~55.3 kDa.
- Application
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Alternative Names
T4 ligase, T4 phage (His)
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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AA Sequence
MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYYIKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVLRRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGARCFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQVKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVWDYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQGLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGKIKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLPIAIRLREDKTKANTFEDVFGDFHEVTGL
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Molecular Weight
55.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
T4 ligase, an enzyme derived from the T4 bacteriophage, plays a crucial role in DNA manipulation and recombinant DNA technology. Its primary function is to catalyze the formation of phosphodiester bonds between adjacent nucleotides, effectively joining DNA fragments. This capability is essential in various molecular biology applications, including cloning, gene assembly, and the construction of recombinant proteins. The study of T4 ligase has gained significant interest due to its high specificity and efficiency in ligating cohesive ends of DNA fragments, which enhances the success rate of cloning procedures. Moreover, T4 ligase operates optimally at a range of temperatures and can work with mismatched ends, making it a versatile tool in genetic engineering. Research has also focused on improving the enzyme's performance through mutagenesis and protein engineering, aiming to create variants with enhanced characteristics for industrial and therapeutic applications. Understanding the structure and function of T4 ligase not only contributes to the field of synthetic biology but also opens doors for innovation in biotechnology, such as the development of novel gene therapies and genetically modified organisms. As researchers continue to explore the potential applications of T4 ligase, its implications for advancing genetic research and biotechnology remain vast and promising.
