Analytical Data
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Gene name
FIS1
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简介
The FIS1 protein is essential for mitochondrial network fragmentation and perinuclear clustering. Although FIS1 plays a minor role in recruiting DNM1L to the mitochondrial surface, it is associated with fission. FIS1 Protein, Human (His) is the recombinant human-derived FIS1 protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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Alternative Names
Mitochondrial Fission 1 Protein; FIS1 Homolog; hFis1; Tetratricopeptide Repeat Protein 11; TPR Repeat Protein 11; FIS1; TTC11; CGI-135
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y3D6
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Expression Region
M1-G122
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Protein Length
Cytoplasmic Domain
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Molecular Weight
15.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FIS1 ( mitochondrial fission 1) is a crucial protein involved in the regulation of mitochondrial dynamics, specifically in the process of mitochondrial fission, which is essential for maintaining cellular health and energy metabolism. Research into FIS1 has gained prominence due to its implications in various biological processes, including apoptosis, cellular proliferation, and inherited diseases. Understanding the mechanisms by which FIS1 interacts with other proteins and influences mitochondrial morphology is vital, as dysfunctional mitochondria have been linked to numerous pathologies, such as neurodegenerative disorders, metabolic syndromes, and cancer. Studies have shown that FIS1 plays a pivotal role in the recruitment of dynamin-related proteins, facilitating the division of mitochondria, and thereby enabling the distribution of mtDNA and organelles during cell division. Furthermore, alterations in FIS1 expression levels can lead to mitochondrial fragmentation, contributing to the onset of disease states. Consequently, researchers are focused on elucidating the regulatory pathways governing FIS1 function and exploring its potential as a therapeutic target. Insights gained from FIS1 research could lead to novel strategies for mitochondrial-related diseases, making it a significant focus in contemporary biomedical research.
