Analytical Data
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Gene name
PIP4K2A
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简介
The PIP4K2A protein phosphorylates phosphatidylinositol 5-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP4K2A Protein, Human (HEK293, His) is the recombinant human-derived PIP4K2A protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48426-1
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Expression Region
M1-T406
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AA Sequence
MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT
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Protein Length
Full Length of Isoform-1
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Molecular Weight
52-58 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIP4K2A, or Phosphatidylinositol 5-phosphate 4-kinase type 2 alpha, is an important enzyme involved in phosphoinositide signaling pathways, which play a crucial role in various cellular processes such as cell growth, metabolism, and membrane trafficking. This protein specifically phosphorylates phosphatidylinositol 5-phosphate (PI5P) to produce phosphatidylinositol 4,5-bisphosphate (PIP2), thereby influencing downstream signaling cascades that are pivotal for cellular responses to external stimuli. Research has shown that dysregulation of PIP4K2A is associated with various diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. The recombinant expression and characterization of PIP4K2A are essential for understanding its biochemical properties and functional mechanisms. Utilizing recombinant protein techniques allows for the production of PIP4K2A in a controlled environment, facilitating the study of its enzymatic activity, interaction with other cellular components, and role in signaling pathways. Moreover, investigating the structure-function relationship of PIP4K2A can provide insights into its regulation and help identify small molecules that can modulate its activity for potential therapeutic applications. As result, detailed studies on PIP4K2A are not only significant for basic biochemical research but also for the development of novel strategies in disease treatment and prevention.
