Analytical Data
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基因名
MKK6
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简介
The MKK6 protein is a dual-specificity kinase involved in the MAP kinase pathway. MKK6 Protein, Human (S207D, T211D, sf9, His) is the recombinant human-derived MKK6 protein, expressed by Sf9 insect cells, with N-6*His labeled tag.
- Application
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生物活性
Measured in a cell proliferation assay using Hep3B cells. The ED50 for this effect is 77.27 ng/mL, corresponding to a specific activity is 1.29×104 units/mg. Measured in a cell proliferation assay using Hep3B cells. The ED50 for this effect is 77.27 ng/mL, corresponding to a specific activity is 1.29×104 units/mg.
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别名
MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3
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种属
Human
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表达系统
Baculovirus
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P52564-1
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表达区间
M1-D334, S207D, T211D
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氨基酸序列
MSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDDVAKDIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD
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蛋白长度
Full Length of Isoform-1
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分子量
40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MKK6, or Mitogen-Activated Protein Kinase Kinase 6, is a pivotal component of the MAPK signaling cascade, which plays a crucial role in cellular responses to various extracellular stimuli, including growth factors, stress, and inflammatory signals. The study of MKK6 has garnered significant attention due to its involvement in regulating key physiological processes such as cell proliferation, differentiation, and apoptosis. Dysregulation of MKK6 and its downstream effectors, particularly the p38 MAPK pathway, has been implicated in various diseases, including cancer and inflammatory disorders. Researchers aim to understand the precise mechanisms through which MKK6 influences cellular functions and its potential as a therapeutic target. Recombinant MKK6 protein is essential for these studies, as it allows scientists to examine the protein's biochemical properties, interactions, and effects in a controlled environment. By expressing and purifying MKK6 using advanced recombinant techniques, researchers can explore its structure-function relationships, identify novel regulatory mechanisms, and develop inhibitors that could modulate its activity. This line of research not only enhances our understanding of the MAPK signaling network but also contributes to the development of innovative strategies for managing diseases linked to MKK6 dysregulation. Ultimately, the detailed study of recombinant MKK6 is expected to facilitate the advancement of therapeutic applications aimed at restoring normal signaling pathways in various pathological conditions.
