Analytical Data
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Gene name
TAOK3
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简介
TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His, GST) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells, with N-His and N-GST labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase TAO3; JNK/SAPK-inhibitory kinase; DPK; JIK; KDS; MAP3K18
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H2K8-1
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Expression Region
M1-D411
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Protein Length
Partial
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Molecular Weight
85 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TAOK3 (TAO Kinase 3) is a serine/threonine kinase that belongs to the TAO family of proteins, which are known to play significant roles in various cellular processes, including apoptosis, neurogenesis, and the regulation of the cytoskeleton. Recent research has highlighted its involvement in neurodevelopmental disorders and certain forms of cancer, making it a target of interest in both basic and translational biology. Mutations and dysregulation of TAOK3 have been implicated in conditions such as autism spectrum disorders and other neuropsychiatric conditions, suggesting a crucial role in central nervous system development and function. The understanding of TAOK3's function has been limited due to the lack of detailed studies on its structural biology and biochemical properties. The recombinant expression of TAOK3 in various systems offers the potential to elucidate its molecular mechanisms, interactions with upstream and downstream signaling pathways, and its role in disease pathology. By studying the recombinant TAOK3 protein, researchers aim to provide insights into its enzymatic activity, substrate specificity, and potential as a therapeutic target. Overall, the investigation of TAOK3 and its recombinant protein serves as a promising avenue for advancing our understanding of complex biological processes and developing novel therapeutic strategies for related disorders.
