Analytical Data
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基因名
RNF5
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简介
The RNF5 protein is a membrane-bound E3 ubiquitin protein ligase that is critical for ubiquitination of target proteins and can cooperate with UBE2D1/UBCH5A and UBE2D2/UBC4 to achieve ubiquitination over a broad substrate range. Notably, it mediates PXN/paxillin ubiquitination, affecting cell motility and cell positioning. RNF5 Protein, Human (Cell-Free, His) is the recombinant human-derived RNF5 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Application
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别名
E3 ubiquitin-protein ligase RNF5; RING finger protein 5; Ram1 homolog; HsRma1
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种属
Human
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表达系统
E. coli Cell-free
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标签
N-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99942
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表达区间
A2-I180
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蛋白长度
Full Length of Mature Protein
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分子量
27 kDa&54 kDa&108 kDa. It is speculated that the protein forms a multimer structure.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF5, a RING-type E3 ubiquitin ligase, plays a crucial role in various cellular processes, including protein degradation, immune response, and signal transduction. Research has shown that RNF5 is involved in the regulation of the NF-κB signaling pathway, which is essential for inflammatory responses and immune system functions. Dysregulation of RNF5 has been linked to several diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. The study of recombinant RNF5 proteins has gained attention, as these proteins can be utilized to better understand the mechanistic pathways influenced by RNF5 and to explore its substrate interactions. Additionally, by producing and analyzing recombinant RNF5, researchers can develop assays to evaluate the effects of potential inhibitors or modulators, paving the way for novel therapeutic strategies. Overall, the investigation of RNF5 recombinant proteins not only enhances our understanding of its biological functions but also contributes to the development of innovative treatments for related diseases.
