Analytical Data
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Gene name
RNF5
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简介
The RNF5 protein is a membrane-bound E3 ubiquitin protein ligase that is critical for ubiquitination of target proteins and can cooperate with UBE2D1/UBCH5A and UBE2D2/UBC4 to achieve ubiquitination over a broad substrate range. Notably, it mediates PXN/paxillin ubiquitination, affecting cell motility and cell positioning. RNF5 Protein, Human (Cell-Free, His) is the recombinant human-derived RNF5 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Application
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Alternative Names
E3 ubiquitin-protein ligase RNF5; RING finger protein 5; Ram1 homolog; HsRma1
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Species
Human
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Source
E. coli Cell-free
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99942
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Expression Region
A2-I180
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Protein Length
Full Length of Mature Protein
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Molecular Weight
27 kDa&54 kDa&108 kDa. It is speculated that the protein forms a multimer structure.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF5, a RING-type E3 ubiquitin ligase, plays a crucial role in various cellular processes, including protein degradation, immune response, and signal transduction. Research has shown that RNF5 is involved in the regulation of the NF-κB signaling pathway, which is essential for inflammatory responses and immune system functions. Dysregulation of RNF5 has been linked to several diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. The study of recombinant RNF5 proteins has gained attention, as these proteins can be utilized to better understand the mechanistic pathways influenced by RNF5 and to explore its substrate interactions. Additionally, by producing and analyzing recombinant RNF5, researchers can develop assays to evaluate the effects of potential inhibitors or modulators, paving the way for novel therapeutic strategies. Overall, the investigation of RNF5 recombinant proteins not only enhances our understanding of its biological functions but also contributes to the development of innovative treatments for related diseases.
