Analytical Data
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Gene name
GJA1
- Application
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Alternative Names
Connexin-43 Short name: Cx43 Gap junction 43KDA heart protein
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P17302
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Expression Region
233-382aa
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Molecular Weight
20.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GJA1, also known as connexin 43 (Cx43), is a pivotal protein involved in intercellular communication through gap junctions, facilitating the direct transfer of ions and small molecules between adjacent cells. Its role is crucial in various physiological processes, including cardiac and skeletal muscle function, as well as in embryonic development. Research has indicated that aberrations in GJA1 expression or function can lead to several pathologies, including cardiac arrhythmias, vascular diseases, and even certain types of cancer. In the context of regenerative medicine, GJA1 has attracted attention for its potential to enhance tissue repair and regeneration, especially in heart tissues post-infarction. Recombinant GJA1 proteins can be used in studies to elucidate the mechanisms of gap junction intercellular communication and to investigate how alterations in this communication can affect cellular behavior in health and disease. Moreover, producing GJA1 as a recombinant protein allows for controlled studies on its functional properties, interactions with other proteins, and its implications in cell signaling pathways. This research is vital for developing potential therapeutic strategies aimed at restoring normal gap junction communication in diseased tissues and advancing our understanding of cellular communication's role in health and disease. Therefore, the study of GJA1 recombinant proteins represents a significant area of investigation in cellular biology and therapeutic development.
