Analytical Data
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Gene name
ROCK1
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简介
The ROCK1 protein is a key kinase that regulates the actin cytoskeleton, cell polarity, smooth muscle contraction, and multiple cellular functions. It controls stress fibers, focal adhesion formation, neurite retraction, and cell motility by phosphorylating substrates such as DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1, and PPP1R12A. ROCK1 Protein, Human (sf9, His) is the recombinant human-derived ROCK1 protein, expressed by sf9 insect cells , with N-8*His labeled tag.
- Application
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Alternative Names
ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
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Species
Human
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Source
Baculovirus
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Tag
N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13464
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Expression Region
S6-R415
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Protein Length
Partial
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Molecular Weight
50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ROCK1, or Rho-associated protein kinase 1, is a serine/threonine kinase that plays a significant role in various cellular processes, including cell morphology, motility, and proliferation, by mediating signaling pathways activated by Rho GTPases. Its involvement in cytoskeletal dynamics and cell adhesion has garnered attention in the context of several diseases, including cancer, cardiovascular disorders, and neurological diseases. Research on ROCK1 has revealed that its overexpression or dysregulation can contribute to the invasive and metastatic potential of cancer cells, making it a potential therapeutic target. Additionally, ROCK1 has been implicated in smooth muscle contraction and vascular function, offering insights into its role in hypertension and other vascular complications. The recombinant production of ROCK1 protein has facilitated detailed studies of its structure and function, allowing researchers to explore its catalytic mechanisms and interactions with downstream effectors. These studies are crucial for understanding the pathological processes in which ROCK1 is involved and for developing targeted therapies that can modulate its activity. Consequently, ROCK1 is emerging as a critical component in the field of cell signaling and therapeutic development.
