Analytical Data
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Gene name
TNIK
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简介
TNIK protein is a key activator of the Wnt signaling pathway and regulates gene expression by phosphorylating TCF4/TCF7L2 at the promoter of Wnt target genes. TNIK is located upstream of the JUN N-terminal pathway and contributes to the complex Wnt signaling cascade. TNIK Protein, Human (sf9, His) is the recombinant human-derived TNIK protein, expressed by sf9 insect cells , with N-8*His labeled tag.
- Application
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Alternative Names
TNIK; TRAF2 and NCK-interacting protein kinase
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Species
Human
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Source
Baculovirus
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Tag
N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKE5
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Expression Region
D11-G314
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Protein Length
Partial
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Molecular Weight
36.8 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TNIK (TRAF2-and-NCK-interacting protein kinase) is a member of the CNK family of proteins that plays a crucial role in various cellular processes, including signal transduction, cytoskeletal organization, and transcriptional regulation. Its involvement in the Wnt signaling pathway, which is pivotal in development and oncogenesis, has drawn significant research attention. Studies have demonstrated that TNIK interacts with various key signaling molecules, influencing pathways relevant to cancer progression and neurological disorders. The dysregulation of TNIK expression and activity has been associated with pathological conditions, making it a potential therapeutic target. Recent advancements in structural biology have enabled researchers to gain insights into its functional domains and mechanisms of action, paving the way for the development of TNIK inhibitors. Consequently, the exploration of TNIK as a therapeutic target has gained momentum, aiming to elucidate its role in disease and to potentially develop novel treatments by manipulating its activity in signaling pathways.
