Analytical Data
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基因名
COASY
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简介
COASY protein is a bifunctional enzyme that plays a key role in catalyzing the fourth and fifth steps of the CoA biosynthetic pathway. The coaD domain promotes the phosphopantothenate adenylyltransferase activity of the fourth reaction, and the coaE domain drives the dephosphoCoA kinase activity of the fifth reaction. COASY Protein, Human (His) is the recombinant human-derived COASY protein, expressed by E. coli , with N-His labeled tag.
- Application
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生物活性
Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is typically 15.42 ng/mL, corresponding to a specific activity is 64850.843 units/mg. Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is typically 15.42 ng/mL, corresponding to a specific activity is 64850.843 units/mg.
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别名
Bifunctional coenzyme A synthase; CoA synthase; NBP; POV-2; PPAT; DPCK
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q13057-1
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表达区间
M296-D564
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氨基酸序列
MAINRFRLENDLEELALYQIQLLKDLRHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD
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蛋白长度
Partial
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分子量
32 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COASY (Coenzyme A Synthase), an essential enzyme in the biosynthesis of coenzyme A (CoA), has garnered significant attention in recent years due to its critical role in cellular metabolism and energy production. CoA is vital for various biochemical pathways, including fatty acid metabolism, the citric acid cycle, and the synthesis and degradation of ketone bodies. Its deficiency is associated with numerous metabolic disorders, leading researchers to investigate COASY as a potential therapeutic target. Despite its importance, the structural and functional properties of COASY remain poorly understood. Recent advancements in recombinant protein technology have enabled the expression and purification of COASY, allowing for in-depth studies of its enzymatic mechanisms, interactions with other cellular components, and potential regulation pathways. Understanding the function and regulation of COASY could pave the way for novel therapeutic strategies to combat metabolic diseases linked to CoA deficiency. Furthermore, insights gained from COASY research may also contribute to the broader field of metabolism and mitochondrial function, highlighting the enzyme's significance beyond its immediate biochemical roles. Overall, the study of COASY as a recombinant protein represents a promising avenue for elucidating the complexities of cellular metabolism and identifying new approaches to treat related disorders.
