Analytical Data
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Gene name
ART1
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简介
ART1 Protein is an arginine-specific ADP-ribosyltransferase. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. ART1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ART1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×10^4 Unit/mg. Measured in a cell proliferation assay using A549 cells.The ED50 for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×104 Unit/mg.
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Alternative Names
NAD(P)(+)--arginine ADP-ribosyltransferase; Mono(ADP-ribosyl)transferase
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Species
Cynomolgus
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
A0A2K5WCR3
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Expression Region
Q23-N294
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AA Sequence
QSHPITRRDLFSQEMPLDMALASFDDQYAGCAAAMTAALPDLNHTEFQANKVYADGWTLASSQWQERQAWGPEWSLSPTRPPPPPLGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLGRGQRPPRCHQVFRGVHGLHFRPAGPGATVRLGGFASASLKNVAAQQFGEDTFFGIWTCLGAPIKGYSFFPGEEEVLIPPFETFQVINASRPAQGPARIYLRALGKRSTYNCEYIKDKKCKSGPCHLDN
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Protein Length
Partial
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Molecular Weight
33-34 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ART1, also known as antigen presentation receptor 1, is a crucial protein in the field of immunology, particularly in understanding how the immune system recognizes and responds to foreign antigens. Research into ART1 has gained momentum due to its role in T-cell activation and its potential implications in various diseases, including autoimmune disorders and cancers. The ART1 protein functions as a co-stimulatory receptor on antigen-presenting cells, enhancing the activation and proliferation of T cells upon interaction with specific ligands. This function is vital for the initiation of adaptive immune responses. In the context of cancer immunotherapy, targeting ART1 and its signaling pathways has emerged as a promising strategy to enhance anti-tumor immunity. Moreover, recent studies have linked variations in ART1 expression to the severity of autoimmune diseases, making it a potential biomarker for disease progression. As researchers continue to unravel the intricate mechanisms by which ART1 influences immune responses, there is a growing interest in developing ART1-targeted therapeutics that could improve patient outcomes in various immunological conditions. Understanding ART1's structure and function at the molecular level is imperative for these advancements, laying the groundwork for novel strategies to modulate immune responses in health and disease.
