Analytical Data
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Gene name
PLGF-1
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简介
The PLGF-1 protein is a key growth factor for angiogenesis and endothelial cell growth, crucially stimulating cell proliferation and migration by binding to the FLT1/VEGFR-1 receptor. It coordinates the angiogenic process, regulates blood vessel growth, and exhibits additional binding capabilities to NRP1/neuropilin-1 and NRP2/neuropilin-2. PLGF-1 Protein, Human (N-His) is the recombinant human-derived PLGF-1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using HUVEC Human umbilical vein endothelial cells. The ED50 this effect is 20.22 ng/mL, corresponding to a specific activity is 4.95×104 units/mg. Measured in a cell proliferation assay using HUVEC Human umbilical vein endothelial cells. The ED50 for this effect is 20.22 ng/mL, corresponding to a specific activity is 4.95×104 units/mg.
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Alternative Names
Placenta growth factor; PlGF; PGF; PGFL
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P49763-2
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Expression Region
L19-R149
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Protein Length
Partial
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLGF-1 (placental growth factor-1) is a member of the vascular endothelial growth factor (VEGF) family, primarily involved in angiogenesis and vascular development. Research on PLGF-1 has garnered attention due to its significant role in various physiological and pathological processes, including tumor progression, cardiovascular diseases, and wound healing. Elevated levels of PLGF-1 have been associated with several malignancies, indicating its potential as a biomarker and therapeutic target. Furthermore, studies have shown that PLGF-1 can enhance the survival and proliferation of endothelial cells, thus contributing to neovascularization. The understanding of PLGF-1's mechanisms and functions has advanced through the development of recombinant protein technologies, enabling its application in experimental therapies and clinical research. Investigating PLGF-1 not only helps elucidate its biological roles but also opens avenues for innovative treatments aimed at modulating angiogenesis and addressing diseases with vascular implications. With ongoing studies focusing on its signaling pathways and interactions, PLGF-1 continues to emerge as a promising candidate in the fields of regenerative medicine and oncology.
