Analytical Data
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基因名
FAM20B
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简介
The FAM20B protein plays a key role in regulating mature glycosaminoglycan (GAG) chains by catalyzing xylose 2-O-phosphorylation in the glycosaminoglycan-protein linking region of the proteoglycan. This process is critical for the synthesis of sulfated GAGs (such as heparan sulfate and chondroitin sulfate) on the common GAG-protein linking region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of the core protein. FAM20B Protein, Human (HEK293, Fc) is the recombinant human-derived FAM20B protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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生物活性
Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 unit/mg. Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 Unit/mg.
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别名
Glycosaminoglycan xylosylkinase; Xylose kinase; KIAA0475
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种属
Human
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表达系统
HEK293
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标签
N-hFc
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O75063
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表达区间
S31-L409
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氨基酸序列
SAANREDQRAFHRMMTGLRVELAPKLDHTLQSPWEIAAQWVVPREVYPEETPELGAVMHAMATKKIIKADVGYKGTQLKALLILEGGQKVVFKPKRYSRDHVVEGEPYAGYDRHNAEVAAFHLDRILGFHRAPLVVGRFVNLRTEIKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDIMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDAVDQRLLSVLATVKQCTDQFGMDTVLVEDRMPLSHL
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蛋白长度
Partial
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分子量
75-80 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FAM20B, a member of the FAM20 gene family, plays a critical role in bone and dental development through its involvement in the regulation of the glycosylation of proteins. It encodes a protein that functions as a kinase, which phosphorylates specific serine residues in the secreted protein matrix, a process essential for the proper mineralization of bones and teeth. Mutations in FAM20B have been linked to various skeletal disorders, including amelogenesis imperfecta, a genetic condition affecting tooth enamel formation. The study of recombinant FAM20B proteins is vital for understanding the molecular mechanisms underlying these conditions and their role in initiating mineralization processes. Researchers are increasingly focused on producing recombinant forms of FAM20B to investigate its enzymatic activity, substrate specificity, and interaction with other biomolecules. This research can provide insights into the pathophysiological mechanisms of diseases associated with FAM20B mutations and contribute to the development of potential therapeutic strategies aimed at enhancing bone and dental health. Understanding the functional properties of FAM20B may also pave the way for new biomaterials in regenerative medicine and tissue engineering applications. Hence, developing effective recombinant protein systems to study FAM20B's function and its implications for human health remains a pivotal area of research in molecular and cell biology.
