Analytical Data
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Gene name
FAM20B
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简介
The FAM20B protein plays a key role in regulating mature glycosaminoglycan (GAG) chains by catalyzing xylose 2-O-phosphorylation in the glycosaminoglycan-protein linking region of the proteoglycan. This process is critical for the synthesis of sulfated GAGs (such as heparan sulfate and chondroitin sulfate) on the common GAG-protein linking region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of the core protein. FAM20B Protein, Human (HEK293, Fc) is the recombinant human-derived FAM20B protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 unit/mg. Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 Unit/mg.
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Alternative Names
Glycosaminoglycan xylosylkinase; Xylose kinase; KIAA0475
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Species
Human
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O75063
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Expression Region
S31-L409
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AA Sequence
SAANREDQRAFHRMMTGLRVELAPKLDHTLQSPWEIAAQWVVPREVYPEETPELGAVMHAMATKKIIKADVGYKGTQLKALLILEGGQKVVFKPKRYSRDHVVEGEPYAGYDRHNAEVAAFHLDRILGFHRAPLVVGRFVNLRTEIKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDIMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDAVDQRLLSVLATVKQCTDQFGMDTVLVEDRMPLSHL
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Protein Length
Partial
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Molecular Weight
75-80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FAM20B, a member of the FAM20 gene family, plays a critical role in bone and dental development through its involvement in the regulation of the glycosylation of proteins. It encodes a protein that functions as a kinase, which phosphorylates specific serine residues in the secreted protein matrix, a process essential for the proper mineralization of bones and teeth. Mutations in FAM20B have been linked to various skeletal disorders, including amelogenesis imperfecta, a genetic condition affecting tooth enamel formation. The study of recombinant FAM20B proteins is vital for understanding the molecular mechanisms underlying these conditions and their role in initiating mineralization processes. Researchers are increasingly focused on producing recombinant forms of FAM20B to investigate its enzymatic activity, substrate specificity, and interaction with other biomolecules. This research can provide insights into the pathophysiological mechanisms of diseases associated with FAM20B mutations and contribute to the development of potential therapeutic strategies aimed at enhancing bone and dental health. Understanding the functional properties of FAM20B may also pave the way for new biomaterials in regenerative medicine and tissue engineering applications. Hence, developing effective recombinant protein systems to study FAM20B's function and its implications for human health remains a pivotal area of research in molecular and cell biology.
