Analytical Data
-
基因名
Arylsulfatase B/ARSB
- Application
-
别名
ASB; G4S; N-acetylgalactosamine-4-sulfatase
-
种属
Human
-
表达系统
E. coli
-
标签
N-His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P15848
-
表达区间
Leu81~Met533
-
分子量
55kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Arylsulfatase B (ARSB) is an important enzyme involved in the degradation of glycosaminoglycans, specifically heparan sulfate and dermatan sulfate. Mutations in the ARSB gene lead to the accumulation of these substrates, resulting in a lysosomal storage disorder known as mucopolysaccharidosis type VI (MPS VI). This disorder is characterized by a range of symptoms including skeletal dysplasia, cardiac issues, and progressive neurological impairment. The importance of ARSB in cellular metabolism has spurred significant research into its structure, function, and therapeutic potential. Recombinant ARSB protein has been developed to explore possible enzyme replacement therapies for MPS VI, aiming to alleviate the symptoms by restoring normal enzyme activity. The successful expression and purification of functional ARSB are critical for both basic research and clinical applications, which could lead to improved treatment options for affected individuals. Thus, understanding the biochemical properties and activity of ARSB is essential for advancing therapeutic strategies and enhancing patient outcomes in lysosomal storage disorders.
