Analytical Data
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Gene name
SMPD2
- Application
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Alternative Names
SMPD2; N-SMase; ISC1; Neutral Sphingomyelin Phosphodiesterase; Sphingomyelin Phosphodiesterase 2,Neutral Membrane; Lyso-platelet-activating factor-phospholipase C
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Species
Rat
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9ET64
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Expression Region
Met1~Ala324
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Molecular Weight
67kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SMPD2, or sphingomyelin phosphodiesterase 2, is an important enzyme that plays a crucial role in sphingolipid metabolism, specifically in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. This enzymatic activity is vital for maintaining cellular membrane integrity and regulating various cellular processes, including apoptosis, cell signaling, and inflammation. Dysregulation of SMPD2 has been implicated in various diseases, including neurodegenerative disorders, cancer, and cardiovascular diseases. The study of SMPD2 recombinant protein has gained traction in recent years as researchers aim to understand its structure-function relationships and biochemical properties. Recombinant expression systems allow for the production of the SMPD2 protein in sufficient quantities for in-depth analysis, including enzymatic assays, structural studies using techniques like X-ray crystallography or NMR, and investigations into its interaction with other cellular components. By exploring the function and regulatory mechanisms of SMPD2, researchers hope to uncover therapeutic targets that could mitigate the effects of diseases linked to sphingolipid metabolism dysfunction. As such, the development and characterization of SMPD2 recombinant protein is an essential step towards identifying potential drug candidates and advancing our understanding of its role in health and disease.
