Analytical Data
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基因名
PKCi
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简介
PKCi protein has adenosine 5'-monophosphate amidase activity and can hydrolyze compounds such as AMP-NH2. It also acts on AMP-morpholidate, GMP-morpholidate, lysyl-AMP, Met-AMP, His-AMP, Asp-AMP, lysyl-GMP and AMP-N-alanine methyl ester. PKCi Protein, Human (H114A, His) is the recombinant human-derived PKCi protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
HINT1; Adenosine 5'-monophosphoramidase HINT1; Desumoylating isopeptidase HINT1; Histidine triad nucleotide-binding protein 1; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49773
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表达区间
M1-G126, H114A
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PKCi, or Protein Kinase C iota, is a member of the PKC family of serine/threonine kinases, which play crucial roles in various cellular processes, including cell growth, differentiation, and apoptosis. The study of PKCi has gained significant attention due to its involvement in cancer progression and metastasis. Research indicates that PKCi is often overexpressed in several cancer types, such as breast, prostate, and pancreatic cancers, leading to enhanced cell survival and proliferation. Understanding the molecular mechanisms by which PKCi contributes to tumorigenesis is imperative for the development of targeted therapies. Researchers focus on elucidating PKCi's signaling pathways to identify potential therapeutic targets that could inhibit its oncogenic functions. Additionally, the generation of recombinant PKCi proteins has allowed for detailed studies of its structure and enzymatic activity, facilitating high-throughput screening of small molecules that could modulate its activity. By studying PKCi in various cellular contexts, scientists aim to uncover its specific roles in cancer biology and its potential as a biomarker for cancer diagnosis and prognosis. The ongoing research on PKCi not only enhances our understanding of its contributions to malignancy but also opens up new avenues for the design of innovative cancer therapeutics that can improve clinical outcomes for patients affected by PKCi-associated tumors.
