Analytical Data
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Gene name
PDE4D
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简介
PDE4D (Phosphodiesterase 4D) protein, as a hydrolytic enzyme, degrades the second messenger cAMP, a crucial regulator in various physiological processes. Its enzymatic activity in breaking down cAMP underscores PDE4D's pivotal role in modulating cellular signaling cascades, maintaining the intricate balance of intracellular communication mediated by this critical second messenger. PDE4D Protein, Human is the recombinant human-derived PDE4D protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
PDE4D; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4D; DPDE3; PDE43
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08499
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Expression Region
T388-S715
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDE4D (phosphodiesterase 4D) is an enzyme that plays a crucial role in the regulation of intracellular signaling by hydrolyzing cyclic adenosine monophosphate (cAMP), which is a key second messenger involved in various physiological processes, including cellular growth, differentiation, and inflammatory responses. The importance of PDE4D has gained attention due to its involvement in several pathological conditions such as asthma, chronic obstructive pulmonary disease (COPD), and neurological disorders. Research has shown that dysregulation of PDE4D activity can lead to heightened inflammation and impaired cellular signaling, making it a potential therapeutic target. The recombinant protein of PDE4D is particularly valuable for studying its enzymatic properties, interaction with other signaling molecules, and role in disease mechanisms. It can be produced using various expression systems, allowing for large-scale production to facilitate biochemical assays and drug screening. Additionally, understanding the structure-function relationship of PDE4D through recombinant forms can aid in the design of selective inhibitors, which may serve as effective treatments for conditions associated with abnormal PDE4D activity. The ongoing research on PDE4D recombinant protein thus holds promise for developing novel therapeutic strategies to mitigate the impact of diseases linked to cAMP regulation and offers insights into the enzyme's multifaceted roles in cellular processes.
