Analytical Data
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Gene name
RNF43
- Application
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Species
Canine
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
XP_038533015
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Expression Region
G24-Y197
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Protein Length
Partial
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Molecular Weight
30-40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RNF43 (Ring Finger Protein 43) is a crucial component in the regulation of Wnt signaling pathways, which are pivotal for various cellular processes, including development, cell proliferation, and differentiation. Dysregulation of Wnt signaling has been implicated in a range of diseases, particularly cancer, where RNF43 mutations or alterations can lead to aberrant pathway activation. RNF43 functions primarily as an E3 ubiquitin ligase, mediating the degradation of Wnt receptors at the plasma membrane, thereby acting as a negative regulator of Wnt signaling. The study of RNF43 recombinant protein has garnered attention due to its potential roles in tumorigenesis and stem cell biology. By producing and characterizing recombinant RNF43, researchers aim to elucidate its structural and functional properties, understand its interactions with Wnt pathway components, and explore its involvement in oncogenesis. This research has implications for developing targeted therapies for cancer treatment, as manipulating RNF43 activity could restore normal Wnt signaling in tumor cells. Additionally, studying RNF43 may provide insights into the fundamental mechanisms of cellular signaling and its contributions to tissue homeostasis and disease progression. Thus, RNF43 represents a significant focus in molecular biology and oncology, with the potential for impactful therapeutic applications.
