Analytical Data
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Gene name
METTL14-METTL3
- Application
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Alternative Names
(Methyltransferase-like protein 3)(hMETTL3)(N6-adenosine-methyltransferase 70 kDa subunit)(MT-A70)
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86U44
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Expression Region
2-580aa
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Molecular Weight
70.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The METTL14-METTL3 complex is a key player in the process of N6-methyladenosine (m6A) methylation, an important post-transcriptional modification that regulates various aspects of RNA metabolism, including splicing, stability, translation, and degradation. This modification has been implicated in various biological processes, including development, differentiation, and response to stress. Abnormalities in m6A methylation have been associated with several diseases, particularly cancer, where they can influence tumorigenesis and metastasis. Understanding the molecular mechanisms underlying the METTL14-METTL3 complex's function is crucial for unraveling the complexities of m6A regulation. Research on recombinant METTL14-METTL3 proteins aims to elucidate their structural properties, enzymatic activities, and interactions with RNA substrates and other cofactors. By characterizing these aspects, scientists hope to develop targeted therapeutic strategies to modulate m6A methylation and its downstream effects, providing insights into potential interventions for diseases characterized by dysregulated RNA methylation.
