Analytical Data
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基因名
Short chain dehydrogenase
- Application
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别名
Lentilactobacillus buchneri subsp. silagei CD034; Oxidoreductase
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种属
Others
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表达系统
E. coli
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标签
His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
J9VYK8
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表达区间
M1-W248
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Short chain dehydrogenases (SCDs) represent a diverse family of enzymes that play crucial roles in various biological processes, including metabolism, steroidogenesis, and the detoxification of drug and xenobiotic compounds. These enzymes are capable of catalyzing the oxidation or reduction of a variety of substrates, such as alcohols, aldehydes, and ketones, utilizing NAD(P)+ as a cofactor. Due to their significant physiological and biomedical implications, including their involvement in metabolic disorders and cancer, characterizing SCDs at the molecular level is essential. The study of recombinant SCD proteins allows researchers to elucidate their structure-function relationships, investigate their enzymatic mechanisms, and explore their potential applications in biotechnology and pharmaceuticals. By employing techniques such as gene cloning, expression in heterologous systems, and subsequent purification, scientists can produce sufficient quantities of these enzymes for detailed biochemical and biophysical studies. Additionally, understanding the regulatory elements and substrate specificity of SCDs can aid in the development of targeted therapeutics and biocatalysts for industrial processes. Overall, the research on recombinant short chain dehydrogenases fosters a deeper understanding of their roles in health and disease, paving the way for innovative approaches to tackle metabolic and therapeutic challenges.












