Analytical Data
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Gene name
QPRTase
- Application
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Alternative Names
Nicotinate-Nucleotide Pyrophosphorylase [Carboxylating]; Quinolinate Phosphoribosyltransferase [Decarboxylating]; QAPRTase; QPRTase; QPRT
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAH05060.1
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Expression Region
M1-H297
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AA Sequence
MDAEGLALLLPPVTLAALVDSWLREDCPGLNYAALVSGAGPSQAALWAKSPGILAGQPFFDAIFTQLNCQVSWFLPEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH
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Protein Length
Full Length
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Molecular Weight
34.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on QPRTase (quinolinate phosphoribosyltransferase) recombinant proteins has garnered significant attention due to its crucial role in the de novo synthesis of NAD (nicotinamide adenine dinucleotide) via the kynurenine pathway. QPRTase catalyzes the conversion of quinolinate, an intermediate in tryptophan metabolism, into NAD precursors. This enzyme is essential for maintaining NAD levels, which are vital for various cellular processes, including energy metabolism, DNA repair, and cell signaling. Abnormalities in NAD metabolism have been implicated in several diseases, such as neurodegenerative disorders and certain cancers. Therefore, studying recombinant QPRTase is critical for understanding its enzymatic mechanisms, developing potential therapeutic interventions, and exploring its role in physiological and pathological contexts. The ability to produce and study QPRTase in vitro allows for detailed kinetic analyses and provides insights into its structure-function relationships. Furthermore, this research could pave the way for novel strategies to manipulate NAD metabolism for therapeutic purposes, making it a valuable focus in biochemistry and clinical research.
