Analytical Data
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Gene name
Histone-H2B
- Application
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Alternative Names
H2BFL; HIST1H2BC
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Species
Xenopus laevis
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A6I8QAK0
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Expression Region
M1-K126
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Histone H2B is a crucial component of the nucleosome, playing a significant role in the structural organization of eukaryotic chromatin. As one of the four core histones, H2B undergoes various post-translational modifications, which are essential for regulating gene expression, DNA repair, and cell cycle progression. Its dynamic nature makes it a key player in chromatin remodeling and epigenetic regulation. Research on recombinant H2B protein has gained momentum due to its importance in elucidating the mechanisms of chromatin dynamics and the influence of histone modifications on cellular processes. Furthermore, studies involving recombinant H2B, especially in the context of tagged versions for visualization or isolation, have advanced our understanding of histone interactions and the impact of various modifications on chromatin structure and function. This research is particularly relevant for insights into the epigenetic landscape of diseases such as cancer, where aberrations in histone modifications can lead to altered gene expression patterns and contribute to tumorigenesis. Thus, developing recombinant H2B proteins serves not only fundamental biological research but also potential therapeutic approaches in epigenetics.
