Analytical Data
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基因名
Kanamycin kinase type II/NEO
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简介
The Kanamycin kinase type II/NEO protein imparts resistance to aminoglycoside antibiotics, including kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and gentamicin B. It counteracts their inhibitory effects, showcasing the protein's versatility in mitigating the impact of various antibiotics and enabling the organism to thrive in their presence. Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae is the recombinant Kanamycin kinase type II/NEO protein, expressed by E. coli , with tag free.
- Application
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别名
rKlAminoglycoside 3'-phosphotransferase; Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
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种属
Others
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P00552
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表达区间
M1-F264
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氨基酸序列
MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
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蛋白长度
Full Length
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分子量
26-30 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Kanamycin kinase type II (APH(3')-II) is an important enzymatic protein that exhibits resistance to aminoglycoside antibiotics, particularly kanamycin. This enzyme modifies and inactivates the antibiotic, thereby contributing to the bacterial resistance mechanisms that pose significant challenges in treating infectious diseases. The study of Kanamycin kinase type II is particularly relevant in the context of increasing antimicrobial resistance, which has become a global health concern. Understanding the biochemical pathways and structural characteristics of this enzyme provides insights into how bacteria adapt and survive in the presence of antibiotics. Furthermore, the recombinant expression of Kanamycin kinase type II allows researchers to explore its enzymatic properties and develop potential inhibitors that could restore the efficacy of existing antibiotics. The research can also contribute to the design of novel antimicrobial agents by targeting the mechanisms of resistance, enhancing our ability to combat resistant strains of bacteria effectively. Overall, the investigation of Kanamycin kinase type II/NEO recombinant protein represents a critical frontier in microbiological and pharmaceutical research, aiming to address the pressing issue of antibiotic resistance and improve therapeutic options in clinical settings.
