Analytical Data
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Gene name
Kanamycin kinase type II/NEO
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简介
The Kanamycin kinase type II/NEO protein imparts resistance to aminoglycoside antibiotics, including kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and gentamicin B. It counteracts their inhibitory effects, showcasing the protein's versatility in mitigating the impact of various antibiotics and enabling the organism to thrive in their presence. Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae is the recombinant Kanamycin kinase type II/NEO protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
rKlAminoglycoside 3'-phosphotransferase; Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
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Species
Others
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P00552
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Expression Region
M1-F264
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AA Sequence
MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
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Protein Length
Full Length
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Molecular Weight
26-30 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Kanamycin kinase type II (APH(3')-II) is an important enzymatic protein that exhibits resistance to aminoglycoside antibiotics, particularly kanamycin. This enzyme modifies and inactivates the antibiotic, thereby contributing to the bacterial resistance mechanisms that pose significant challenges in treating infectious diseases. The study of Kanamycin kinase type II is particularly relevant in the context of increasing antimicrobial resistance, which has become a global health concern. Understanding the biochemical pathways and structural characteristics of this enzyme provides insights into how bacteria adapt and survive in the presence of antibiotics. Furthermore, the recombinant expression of Kanamycin kinase type II allows researchers to explore its enzymatic properties and develop potential inhibitors that could restore the efficacy of existing antibiotics. The research can also contribute to the design of novel antimicrobial agents by targeting the mechanisms of resistance, enhancing our ability to combat resistant strains of bacteria effectively. Overall, the investigation of Kanamycin kinase type II/NEO recombinant protein represents a critical frontier in microbiological and pharmaceutical research, aiming to address the pressing issue of antibiotic resistance and improve therapeutic options in clinical settings.
