Analytical Data
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Gene name
kshA
- Application
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Species
Others
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Source
E. coli
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Tag
Strep;His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P71875
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Expression Region
M1-H386
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of kshA recombinant protein is rooted in its significance in microbial metabolism and biochemical applications. KshA, a key enzyme in the degradation of steroid compounds, particularly in the catabolism of steroid hormones, plays a critical role in environmental bioremediation and natural steroid recycling processes. Its function is crucial for microorganisms that utilize steroids as a carbon and energy source. The recombinant production of kshA allows for detailed analysis of its enzymatic properties and substrate specificities, providing insights into its catalytic mechanisms. This research is particularly relevant in the fields of synthetic biology and biotechnology, where there is a growing interest in harnessing microbial enzymes for various industrial applications, including waste treatment and biofuel production. Understanding kshA can also lead to the development of novel approaches for the synthesis of valuable biochemicals. Moreover, studying this protein contributes to our broader understanding of microbial diversity and their metabolic capabilities, highlighting the potential of harnessing natural processes for sustainable development. As research progresses, recombinant kshA may also be explored for its therapeutic potential, given the increasing importance of steroid compounds in pharmaceuticals. Therefore, the exploration of kshA not only sheds light on fundamental biological processes but also opens avenues for innovative applications in environmental and industrial biotechnology.
