Analytical Data
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Gene name
SPT16
- Application
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Alternative Names
FACT complex subunit SPT16; Facilitates chromatin transcription complex subunit SPT16; SPT16; Arabidopsis thaliana; Mouse-ear cress
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Species
Others
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O82491
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Expression Region
L662-L952
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The SPT16 protein, part of the FACT (Facilitates Chromatin Transcription) complex, plays a crucial role in chromatin dynamics and transcription regulation. It facilitates the restructuring of nucleosomes, allowing for the effective progression of RNA polymerase II during transcription. Research into SPT16 has gained traction due to its implications in both cellular processes and disease states, particularly cancer, where dysregulation of transcription machinery can lead to aberrant gene expression. Understanding SPT16's structure and function, as well as its interactions with other components of the FACT complex, is vital for elucidating the mechanisms that govern chromatin accessibility and gene expression. Furthermore, investigations have shown that SPT16 is involved in DNA repair processes, highlighting its importance beyond transcription. Given the multifaceted role of SPT16, studies focusing on its biochemical properties, regulatory mechanisms, and interactions with other transcription factors and chromatin remodelers are critical for developing potential therapeutic targets. The evolving knowledge surrounding SPT16 underscores its potential as a key player in epigenetic regulation and a promising focus for future research aimed at addressing transcription-related pathologies.
