Analytical Data
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Gene name
Transglutaminase 3/TGM3
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简介
Transglutaminase 3 (TGM3) acts as a catalyst in the formation of calcium-dependent isopeptide cross-links between glutamine and lysine residues in a variety of proteins, while mediating the conjugation of polyamines to proteins. Transglutaminase 3/TGM3 Protein, Human (sf9, His) is the recombinant human-derived Transglutaminase 3/TGM3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Protein-glutamine gamma-glutamyltransferase E; Transglutaminase E; TGase-3
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08188
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Expression Region
A2-E693
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Protein Length
Full Length of Mature Protein
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Molecular Weight
78 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Transglutaminase 3 (TGM3), a member of the transglutaminase enzyme family, plays a crucial role in various biological processes, including skin barrier formation, cell differentiation, and apoptosis. This enzyme catalyzes the cross-linking of proteins through the formation of isopeptide bonds, which is essential in stabilizing the extracellular matrix and maintaining tissue integrity. Dysregulation of TGM3 has been implicated in several skin disorders, such as psoriasis and eczema, highlighting its significance in dermatological research. The recombinant production of TGM3 offers a valuable tool for studying its functional properties and interactions in vitro and in vivo. Furthermore, the availability of high-purity TGM3 can facilitate the development of therapeutic strategies targeting skin diseases. Advances in recombinant DNA technology have enabled the efficient expression and purification of TGM3 in various heterologous systems, paving the way for deeper investigations into its enzymatic mechanisms and potential applications in regenerative medicine and cosmetic formulations. Understanding the structure-function relationship of TGM3 through recombinant protein studies may also enhance our knowledge of its role in cellular processes and pave the way for innovative treatments for skin-related conditions. As such, TGM3 represents a significant area of research not only for its fundamental biological insights but also for its translational potential in addressing skin health issues.
