Analytical Data
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Gene name
MGMT
- Application
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Alternative Names
Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase
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Species
Rat
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24528
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Expression Region
Met1~Gly209
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Molecular Weight
52kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The MGMT (O6-methylguanine-DNA methyltransferase) protein is a critical DNA repair enzyme that plays a vital role in maintaining genomic stability by removing mutagenic lesions from DNA. Specifically, it repairs O6-methylguanine, a common DNA adduct formed by exposure to alkylating agents, which can lead to mispairing during DNA replication and ultimately result in mutations or cell death. Understanding the structural and functional properties of MGMT is essential, as it has implications in cancer therapy, particularly in relation to the effectiveness of alkylating chemotherapy agents like temozolomide. In human tumors, the expression levels of MGMT can significantly influence the response to such treatments, with high levels of MGMT conferring resistance to therapy. Consequently, research into the recombinant expression of MGMT is focused on elucidating its mechanism of action, exploring its role in DNA repair pathways, and developing potential strategies to inhibit its function to enhance the efficacy of chemotherapy. By employing various recombinant protein technologies, scientists aim to generate MGMT for structural studies, enabling a clearer understanding of its enzymatic activity and interactions with DNA and small molecules. This knowledge may pave the way for innovative therapeutic approaches that could overcome MGMT-related drug resistance in cancer treatment, making it a significant focus in both basic and translational research in oncology.
