Analytical Data
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Gene name
DGCR8
- Application
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Alternative Names
C22orf12; DGCRK6
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Species
Human
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Source
E. coli
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Tag
Strep;His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WYQ5-1
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Expression Region
M1-V773
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Protein Length
Full Length of Isoform-1
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DGCR8 is a crucial protein involved in the biogenesis of microRNAs, playing a pivotal role in post-transcriptional gene regulation. It forms a part of the microprocessor complex along with Drosha, where it facilitates the processing of primary microRNA transcripts into precursor microRNAs. The protein is encoded by the DGCR8 gene located in the 22q11.2 locus, which is associated with the DiGeorge Syndrome, a congenital disorder characterized by developmental issues, cardiac defects, and immune deficiencies. Research on DGCR8 has gained momentum due to its implications in various biological processes, including cell proliferation, differentiation, and apoptosis, as well as its potential involvement in cancer and other diseases where microRNA expression is aberrant. Understanding DGCR8's structure and function can provide insights into the regulatory mechanisms of microRNAs and their impact on gene expression. Furthermore, elucidating its role could pave the way for therapeutic strategies aimed at correcting microRNA dysregulation in diseases. Given the significance of microRNAs in numerous cellular functions and disease states, DGCR8 has emerged as a target of interest for developing novel biomarkers and treatment modalities. Thus, studying recombinant DGCR8 can enhance our comprehension of its mechanistic pathways and foster advancements in biomedical research, particularly in the fields of genetics and molecular biology.
