Analytical Data
-
Gene name
Leukotriene A4 Hydrolase/LTA4H
-
简介
The LTA4H protein is a bifunctional zinc metalloenzyme that acts as an epoxide hydrolase to convert leukotriene A4 (LTA4) into proinflammatory leukotriene B4 (LTB4).It also acts as an aminopeptidase, cleaving N-terminal arginine and fighting inflammation, particularly through Pro-Gly-Pro (PGP) cleavage.Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His) is the recombinant mouse-derived Leukotriene A4 Hydrolase/LTA4H protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
-
Alternative Names
Leukemia inhibitory factor; Leukotriene A-4 hydrolase; LTA-4 hydrolase; LTA4H
-
Species
Mouse
-
Source
Baculovirus
-
Tag
C-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P24527
-
Expression Region
M1-D611
-
Protein Length
Full Length
-
Molecular Weight
62 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Leukotriene A4 hydrolase (LTA4H) is a crucial enzyme in the leukotriene biosynthesis pathway, catalyzing the conversion of leukotriene A4 (LTA4) to leukotriene B4 (LTB4), a potent pro-inflammatory mediator involved in various immune responses. Dysregulation of LTA4H and leukotriene signaling has been implicated in numerous inflammatory diseases, including asthma, rheumatoid arthritis, and cardiovascular disorders. The study of recombinant LTA4H protein has gained significance in understanding its enzymatic mechanisms, regulatory functions, and potential as a therapeutic target. Recombinant expression systems allow for the production of LTA4H in sufficient quantities for detailed biochemical and structural analyses. Researchers aim to elucidate the substrate specificity, kinetic parameters, and structural characteristics of LTA4H using techniques such as X-ray crystallography and enzyme assays. Furthermore, investigating LTA4H inhibitors can provide insights into novel anti-inflammatory strategies. Understanding the functional dynamics of LTA4H not only contributes to the fundamental knowledge of leukotriene metabolism but also paves the way for developing targeted therapies aimed at modulating inflammation and treating related diseases.
