Analytical Data
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Gene name
SPOP
- Application
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Alternative Names
Speckle-type POZ protein; HIB homolog 1; Roadkill homolog 1; SPOP; Homo sapiens; Human
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43791
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Expression Region
K28-V168
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPOP (Speckle-type POZ protein) is a key regulator of various biological processes, including protein degradation and cell signaling. Recent studies have highlighted its crucial role in the ubiquitin-proteasome system, where it functions as an E3 ubiquitin ligase, facilitating the ubiquitination of target proteins for degradation. Dysregulation of SPOP has been implicated in several cancers, particularly prostate cancer, as mutations in the SPOP gene can disrupt its normal function, leading to the accumulation of oncoproteins and promoting tumorigenesis. Given its significant involvement in cancer biology, researchers have focused on the structural and functional characterization of SPOP to better understand its mechanisms of action. The study of SPOP recombinant proteins has become essential for elucidating its interactions with various substrates and for developing potential therapeutic strategies aimed at restoring its normal function or targeting its pathways for cancer treatment. Advances in protein engineering and purification techniques have facilitated the generation of high-quality SPOP recombinant proteins, enabling detailed biochemical and biophysical analyses. This research not only provides insights into SPOP's role in cellular homeostasis but also opens avenues for innovative approaches in cancer therapeutics, making it a focal point of current cancer research efforts.
