Analytical Data
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Gene name
KCNJ2
- Application
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Alternative Names
IRK1
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Species
Human
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Source
HEK293
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Tag
His;MBP;Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P63252
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Expression Region
M1-I427
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNJ2, a member of the inward rectifier potassium channel family, plays a crucial role in maintaining cardiac and neuronal excitability by regulating potassium ion flow across cell membranes. Mutations in the KCNJ2 gene have been associated with various genetic disorders, most notably Andersen-Tawil syndrome, characterized by cardiac arrhythmias, periodic paralysis, and dysmorphic features. The study of KCNJ2 recombinant proteins has emerged as a vital avenue for understanding the structure-function relationships of this ion channel and for elucidating the pathophysiological mechanisms underlying KCNJ2-related diseases. By producing and characterizing recombinant KCNJ2 protein, researchers aim to investigate its biophysical properties, ion conductance, and the effects of specific mutations on its function. Such studies not only enhance our comprehension of KCNJ2's role in cellular physiology but also facilitate the development of targeted therapeutic strategies for conditions linked to KCNJ2 dysfunction. Advances in techniques such as X-ray crystallography and cryo-electron microscopy have further propelled this research area, providing insights into the ion channel's architecture and opening new avenues for drug discovery. Overall, the investigation of KCNJ2 recombinant proteins represents a critical intersection of molecular biology, genetics, and medicine, highlighting the importance of ion channels in health and disease.
