Analytical Data
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Gene name
Histone H3.3
- Application
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Alternative Names
H3.3A; H3F3; H3F3A
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Species
Human
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Source
E. coli
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Tag
His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P84243
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Expression Region
M1-A136
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Histone H3.3 is a variant of the conventional histone H3, playing a critical role in gene regulation, chromatin dynamics, and epigenetic modifications. Unlike the canonical histones that are incorporated into DNA during DNA replication, H3.3 is mainly deposited into chromatin in a replication-independent manner, making it essential for processes such as transcriptional activation and the maintenance of active chromatin states. The distinctive incorporation patterns and post-translational modifications of H3.3 are linked to developmental processes, cellular differentiation, and responses to environmental stimuli. Additionally, mutations in the genes encoding H3.3, particularly H3F3A and H3F3B, have been implicated in various cancers, including glioblastomas and pediatric brain tumors, highlighting its significance in oncogenic processes. Understanding the biological functions and molecular mechanisms of H3.3 is crucial for elucidating its role in health and disease, paving the way for potential therapeutic interventions targeting epigenetic regulation. Researchers are utilizing various techniques, including recombinant protein expression and mass spectrometry, to study the functional implications of H3.3 variants and modifications, thus advancing our knowledge of chromatin biology and its influences on gene expression and cellular behavior.
