Analytical Data
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基因名
Calumenin
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简介
Calumenin Protein, Human (HEK293, His), a recombinant human Calumenin produced in HEK293 cells, has a His tag at the N-terminus. Calumenin protein (CP) is located within the endoplasmic reticulum Ca2+ binding proteins. Calumenin as a suppressor of cell migration and tumor metastasis. Calumenin binds to and stabilizes fibulin-1, leading to inactivation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling[1][2].
- Application
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别名
rHuCalumenin, His; Calumenin; Crocalbin; IEF SSP 9302; CALU
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O43852
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表达区间
K20-F315
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氨基酸序列
KPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEFHHHHHH
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蛋白长度
Full Length of Mature Protein
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分子量
43-55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Calumenin is a calcium-binding protein belonging to the EF-hand family, which has garnered interest due to its potential roles in various cellular processes, including calcium signaling, protein folding, and stress responses in the endoplasmic reticulum (ER). Research into Calumenin has been propelled by the understanding that calcium homeostasis is critical for numerous physiological functions, and dysregulation can lead to various diseases, including neurodegenerative disorders and cancers. The emphasis on recombinant Calumenin synthesis has emerged as researchers aim to elucidate its structure-function relationship and cellular mechanisms. By producing Calumenin as a recombinant protein, scientists can investigate its binding properties, interaction with other proteins, and effects on calcium modulation under controlled conditions. This study further extends to exploring how Calumenin impacts cellular stress responses and the overall stability of proteins within the ER, which are crucial for maintaining cellular health. Additionally, recombinant Calumenin can serve as a valuable tool for therapeutic applications, including the development of strategies to mitigate calcium-related disorders. Overall, the research into recombinant Calumenin is pivotal for advancing our understanding of calcium-binding proteins and their implications in both fundamental biology and disease pathogenesis.
