Analytical Data
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基因名
SDC4
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简介
SDC4 protein is a cell surface proteoglycan that cooperates with SDCBP and PDCD6IP to regulate exosome biogenesis.SDC4 exists as a homodimer and engages in important interactions with various intracellular partners.SDC4 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived SDC4 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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生物活性
1.Measured by its binding ability in a functional ELISA.Immobilized human MDK at 10 μg/mL (100 μl/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 μg/mL. 2.Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to mouse Syndecan-4 and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 °C. The ED50 for this effect is 2.984 μg/mL, corresponding to a specific activity is 3.35×102 U/mg. Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to mouse Syndecan-4 and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 °C. The ED50 for this effect is 2.984 μg/mL, corresponding to a specific activity is 3.35×102 U/mg.
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别名
SDC4; Syndecan-4; SYND4; Ryudocan core protein
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种属
Mouse
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O35988
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表达区间
E24-V146
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蛋白长度
Partial
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分子量
50-55 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The SDC4 protein, also known as Syndecan-4, is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, which play critical roles in cell-cell and cell-matrix interactions. SDC4 is primarily involved in signaling processes that regulate cell adhesion, proliferation, migration, and wound healing. Research has shown that SDC4 interacts with various growth factors and extracellular matrix components, influencing pathways like integrin signaling and fibroblast growth factor (FGF) pathways. In particular, its role in the regulation of cell behavior during tissue regeneration has garnered significant attention. Additionally, SDC4's expression levels have been associated with various pathologies, including cancer, where its altered expression can impact tumor metastasis and progression. As a result, the investigation of SDC4's functional attributes and the effects of its recombinant forms could provide valuable insights into therapeutic strategies for both regenerative medicine and cancer treatment. The recombinant SDC4 protein serves as a model for dissecting its biochemical properties and potential applications, making it a focal point in understanding its contributions to cellular dynamics in both health and disease.
