Analytical Data
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Gene name
ISCU
- Application
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Alternative Names
NifU-like N-terminal domain-containing protein NifU-like protein
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H1K1
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Expression Region
1-167aa
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Molecular Weight
45.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the ISCU (Iron-Sulfur Cluster Utilization) recombinant protein is rooted in its essential role in cellular processes, particularly in the assembly of iron-sulfur (Fe-S) clusters, which are critical for various biological functions. ISCU is a key component of the mitochondrial iron-sulfur cluster biosynthetic machinery, responsible for facilitating the integration of iron and sulfur into clusters that are vital for the activity of numerous enzymes involved in cellular respiration and metabolism. Dysfunction in ISCU has been linked to various neurodegenerative disorders and mitochondrial diseases, emphasizing its importance in maintaining cellular health. The production of recombinant ISCU protein allows researchers to study its structural and functional properties in detail, investigate the mechanisms of Fe-S cluster assembly, and explore potential therapeutic strategies. By utilizing advanced techniques such as X-ray crystallography and electron paramagnetic resonance, scientists aim to elucidate the molecular dynamics of ISCU and its interactions with other proteins involved in cluster biosynthesis. This research facilitates a deeper understanding of the pathophysiological implications of ISCU dysfunction and contributes to the development of targeted interventions for related diseases. Through biochemical characterization and functional assays of the recombinant protein, the potential for novel therapeutic applications targeting ISCU-related metabolic pathways is being explored, highlighting the significance of this protein in both basic science and translational research.
