Analytical Data
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Gene name
ROBO4
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简介
ROBO4 protein is a receptor for Slit proteins (especially SLIT2) and plays a crucial regulatory role in angiogenesis and blood vessel patterning. It mediates the inhibition of primary endothelial cell migration by Slit protein and helps regulate the angiogenic process. ROBO4 Protein, Human (HEK293, Fc) is the recombinant human-derived ROBO4 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Roundabout homolog 4; Magic roundabout; ROBO4; UNQ421/PRO3674
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WZ75-1
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Expression Region
Q28-R467
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AA Sequence
QDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKR
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Protein Length
Partial
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Molecular Weight
87.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ROBO4, a member of the Roundabout (ROBO) family of proteins, is a key player in the regulation of angiogenesis and vascular integrity. It is primarily expressed in endothelial cells and is known to mediate critical processes such as cell migration and guidance during vascular development. Research has shown that ROBO4 plays a significant role in modulating the interactions between endothelial cells and their microenvironment, influencing how blood vessels form and maintain their structure. Elevated expression of ROBO4 has been correlated with tumor angiogenesis, making it a potential target for therapeutic intervention in cancer. Additionally, studies indicate that ROBO4 may inhibit excessive vessel permeability, contributing to the maintenance of normal vascular function. Investigating the signaling pathways and molecular mechanisms involving ROBO4 could provide valuable insights into its function in pathological conditions, including cancer and inflammatory diseases. Understanding ROBO4's role in endothelial biology not only advances our knowledge of vascular physiology but also holds promise for developing novel strategies for treating diseases characterized by aberrant angiogenesis.
