Analytical Data
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Gene name
TIE-1
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简介
TIE-1, a transmembrane kinase, modulates TEK/TIE2 activity, actively participating in the intricate regulation of angiogenesis. TIE-1 Protein, Human (HEK293, His) is the recombinant human-derived TIE-1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha V beta 3 is immobilized at 2 µg/mL (100 µL/well) can bind Recombinant Human Tie-1. The ED50 for this effect is 7.813 μg/mL.
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Alternative Names
Tyrosine-Protein Kinase Receptor Tie-1; TIE1; TIE
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35590
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Expression Region
A22-Q760
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AA Sequence
AVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQ
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Protein Length
Partial
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Molecular Weight
90.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TIE-1, or Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1, is a receptor tyrosine kinase primarily implicated in the regulation of angiogenesis and vascular development. Its expression is predominantly observed in endothelial cells and plays a crucial role in the maturation and stability of blood vessels. Research on TIE-1 has gained prominence due to its involvement in various pathological conditions, including cancer, where abnormal angiogenesis facilitates tumor growth and metastasis. The understanding of TIE-1 function is further enhanced by its interaction with other receptors and signaling pathways that govern vascular integrity. Recent studies have focused on elucidating the molecular mechanisms underlying TIE-1 signaling and its regulatory role in endothelial cell function. This has led to insights into how TIE-1 may serve as a potential therapeutic target for diseases characterized by dysfunctional angiogenesis. Moreover, TIE-1's relevance in the context of inflammatory diseases and cardiovascular disorders highlights its significance in both basic and translational research. Given the complexity of TIE-1's interactions and its pivotal role in vascular biology, ongoing investigations aim to develop targeted therapies that modulate TIE-1 activity to improve clinical outcomes in a range of diseases. This makes TIE-1 a compelling subject for research, with the potential to unlock novel therapeutic strategies and enhance our understanding of vascular pathophysiology.
