Analytical Data
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基因名
Mucolipin-1/MCOLN1
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简介
The TRPML1 protein is a nonselective cation channel that regulates membrane trafficking and metal homeostasis. It promotes the release of Ca(2+) in late endosomes and lysosomes, affecting organelle fusion, exocytosis and autophagy. Mucolipin-1/MCOLN1 Protein, Human (Cell-Free, His) is the recombinant human-derived MCOLN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Application
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别名
Mucolipin-1; MG-2; Mucolipidin; Transient receptor potential channel mucolipin 1; TRPML1
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种属
Human
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表达系统
E. coli Cell-free
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标签
N-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9GZU1
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表达区间
M1-N580
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氨基酸序列
MTAPAGPRGSETERLLTPNPGYGTQAGPSPAPPTPPEEEDLRRRLKYFFMSPCDKFRAKGRKPCKLMLQVVKILVVTVQLILFGLSNQLAVTFREENTIAFRHLFLLGYSDGADDTFAAYTREQLYQAIFHAVDQYLALPDVSLGRYAYVRGGGDPWTNGSGLALCQRYYHRGHVDPANDTFDIDPMVVTDCIQVDPPERPPPPPSDDLTLLESSSSYKNLTLKFHKLVNVTIHFRLKTINLQSLINNEIPDCYTFSVLITFDNKAHSGRIPISLETQAHIQECKHPSVFQHGDNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVGFMWRQRGRVISLWERLEFVNGWYILLVTSDVLTISGTIMKIGIEAKNLASYDVCSILLGTSTLLVWVGVIRYLTFFHNYNILIATLRVALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFAAMQAQQGRSSLVWLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGAGAEESELQAYIAQCQDSPTSGKFRRGSGSACSLLCCCGRDPSEEHSLLVN
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Mucolipin-1 (MCOLN1) is a protein encoded by the MCOLN1 gene, which plays a crucial role in the lysosomal function and cellular ion homeostasis. It is a member of the mucolipin family of proteins and is primarily involved in the transport of cations, such as calcium, in and out of lysosomes. Dysfunction of MCOLN1 has been implicated in mucolipidosis type IV, a rare autosomal recessive lysosomal storage disease that leads to severe neurological and ocular abnormalities. Researchers have focused on understanding the structure and function of MCOLN1 to uncover its role in lysosomal dynamics and disease pathology. The recombinant expression of MCOLN1 has been pivotal for these studies, enabling scientists to investigate its ion channel properties, mechanisms of dysfunction, and potential therapeutic targets. By producing and characterizing the recombinant protein, researchers aim to elucidate the molecular basis of mucolipidosis and develop strategies for intervention. This research not only enhances our understanding of lysosomal biology but also provides insights into potential therapeutic approaches for related disorders and advancing gene therapy techniques. Overall, the investigation of MCOLN1 as a recombinant protein serves as a fundamental step towards developing effective treatments for lysosomal storage disorders.
