Analytical Data
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基因名
GMNN
- Application
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别名
Gem; Geminin,DNA Replication Inhibitor
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种属
Human
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表达系统
E. coli
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标签
N- His & GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75496
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表达区间
Met1~Ile209
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分子量
64kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GMNN (Geminin) is a crucial protein involved in the regulation of DNA replication and cell cycle control. Its primary role is to inhibit the licensing of DNA replication origins, thereby preventing re-replication and ensuring that each segment of the genome is replicated only once during the S phase of the cell cycle. Dysregulation of GMNN expression has been linked to various cancers, making it a significant target for therapeutic interventions. In recent years, the study of GMNN and its recombinant forms has gained attention, as researchers aim to elucidate its structural properties and functional mechanisms. By producing GMNN as a recombinant protein, scientists can investigate its interactions with other cell cycle regulators and DNA replication machinery in detail. Understanding these interactions is vital for developing novel cancer treatments that specifically target the pathways influenced by GMNN. Additionally, the recombinant GMNN can be used as a tool in various biochemical assays to explore its role in cellular processes beyond replication, including transcription regulation and response to DNA damage. Thus, the research on GMNN, particularly its recombinant forms, serves as a significant foundation for uncovering critical aspects of cell cycle regulation and its implications in cancer biology.
