Analytical Data
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Gene name
GEMIN6
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简介
The GEMIN6 protein is a component of the SMN complex and is critical for the assembly of small nuclear ribonucleoproteins (snRNPs), which are essential for spliceosome-mediated pre-mRNA splicing. Most spliceosomal snRNPs share a common set of Sm proteins that form a heptameric protein ring on small nuclear RNAs. GEMIN6 Protein, Human is the recombinant human-derived GEMIN6 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
GEMIN6; Gem-associated protein 6; Gemin-6; SIP2
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WXD5
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Expression Region
M1-Q167
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GEMIN6, a member of the Gemin family of proteins, plays a crucial role in the formation and stability of spliceosomal complexes, which are essential for pre-mRNA splicing. This process is vital for gene expression regulation, as it removes non-coding sequences (introns) from pre-mRNA, enabling the generation of mature mRNA for translation into proteins. The interest in GEMIN6 has grown due to its implications in various biological processes and its potential involvement in disease mechanisms, particularly in neurodegenerative disorders like spinal muscular atrophy, where defects in RNA splicing contribute to pathogenesis. Previous research has demonstrated that GEMIN6 interacts with the SMN (Survival Motor Neuron) complex, which is critical for the assembly of small nuclear ribonucleoproteins (snRNPs), a key component of the spliceosome. Understanding the structural and functional characteristics of GEMIN6, including its interactions with other spliceosomal proteins, can provide insights into the regulation of RNA splicing and the molecular basis of related diseases. Recent advances in proteomics and structural biology techniques have enabled researchers to investigate the molecular dynamics of GEMIN6 and its role in the spliceosome more comprehensively, thereby paving the way for potential therapeutic strategies targeting RNA processing pathways. As a result, the study of GEMIN6 is not only essential for elucidating fundamental biological mechanisms but also holds promise for developing innovative treatments for splicing-related disorders.
