Analytical Data
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Gene name
ROBO4
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简介
ROBO4 protein, as a receptor for Slit proteins (especially SLIT2), plays a key role in angiogenesis and blood vessel pattern formation. It mediates the inhibition of primary endothelial cell migration by Slit protein and actively maintains endothelial barrier organization and function. ROBO4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ROBO4 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
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Species
Mouse
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8C310-1
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Expression Region
L28-W480
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Protein Length
Partial
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Molecular Weight
60-70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ROBO4, a member of the roundabout (ROBO) family of genes, plays a crucial role in vascular development and endothelial cell function. It was initially identified for its involvement in axon guidance during neural development, but recent studies have highlighted its significance in the regulation of angiogenesis and vascular permeability. Research indicates that ROBO4 is predominantly expressed in endothelial cells of blood vessels and is vital for maintaining vascular integrity, particularly in pathological conditions such as tumor growth and inflammation. The protein is known to interact with Slit ligands, which modulate various signaling pathways affecting cell adhesion, migration, and survival. Given its pivotal role in vascular biology, ROBO4 has garnered interest as a potential therapeutic target for treating diseases associated with aberrant blood vessel formation, including cancer and diabetic retinopathy. Investigating the structure and function of recombinant ROBO4 protein provides valuable insights into its mechanisms of action, enabling the development of novel therapeutic strategies aimed at manipulating angiogenesis for clinical benefit. Understanding its role in endothelial cell signaling could also pave the way for innovative interventions that enhance tissue regeneration and repair in ischemic diseases. Thus, the research surrounding ROBO4 recombinant protein not only expands our knowledge of vascular biology but also holds promising implications for therapeutic advancements in a variety of clinical settings.
